Asf1a antibody. Validated Applications: WB, ICC/IF, IP.

Asf1a antibody Mar 6, 2014 · To study Asf1a phosphorylation in vivo, we generated antibodies against all sites in Asf1a and Asf1b that had reasonable immunogenic properties (Asf1a pS166, pS175, pS192; Asf1b pS169). Goat Polyclonal ASF1A antibody, Internal (GTX88842). Tested in Western Blot (WB) applications. Species: Human (Homo sapiens). Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen ASF1A antibody (Cell signaling) and ASF1B antibody (Thermo Scientific) was used to check the efficiency of knockdown. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. Fabgennix Anti-ASF1A Polyclonal, Catalog # ASF1A-BIOTIN. ASF1A/B Antibody (4A1/3) is available as both the non-conjugated anti-ASF1A/B antibody form, as well as multiple conjugated forms of anti-ASF1A/B antibody View Mouse Monoclonal anti-ASF1a Antibody (2A5F8) - BSA Free (NBP2-61683). Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation. ASF1A Antibodies detect Anti-silencing function protein 1A, a histone chaperone vital for chromatin assembly. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Mar 13, 2023 · For this, we used the anti‐ASF1A antibody, which has been proven to be effective in ChIP–PCR analyses (Weng et al, 2014). 1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. [5] [6] [7] Function. Tested Reactivity: Human. Highly specific and rigorously validated in-house, ASF1A (C6E10) Rabbit Monoclonal Antibody (CST #2990) is ready to ship. The protein is a key component of a histone donor complex that functions in nucleosome assembly. Previously, we have shown that residues 421–729 are sufficient for binding to the Rabbit anti-Human ASF1A polyclonal antibody (DPABH-08353), manufactured by Creative Diagnostics with high specificity and affinity. Browse primary antibodies for WB, Flow, IHC, ICC/IF, ELISA, IP, and other applications. ️ Low Prices ️ 100% Guarantee There are no reviews for ASF1a Antibody (NBP2-50108AF700). ️ Low Prices ️ 100% Guarantee Jan 28, 2019 · Anti-silencing function 1a (ASF1a) is a histone H3–H4 chaperone isoform involved in chromatin assembling and transcription regulation. Tested in Human. Monoclonal Antibody for studying Asf1a. Apolipoprotein E-knockout (Apoe KO) mice (strain No. 01223 298 875; About Us; Immunoprecipitation: ASF1a Antibody [NBP2-98775] - ASF1a was immunoprecipitated using: Lane A: 0. View Rabbit Polyclonal anti-ASF1a Antibody [Janelia Fluor® 635] (NBP2-98775JF635). HEK293 Immunofluorescence staining of ASF1A in HeLa cells. Buy rabbit polyclonal antibody to ASF1A (A54639). Anti-silencing function 1, S. Applications: WB, IHC and more. Lane 1: Jurkat whole cell lysate. Buy goat polyclonal antibody to ASF1A (A84425). Validated Applications: WB, ICC/IF. Applications: ELISA, WB, IHC. The antibody can be used for immunoassay techniques, such as FC, IF, IHC-Fr, WB. (B) Increase of phospho-S166 in ASF1A on DSBs. Select appropriate ASF1A antibodies for your research by isotype, epitope, applications and species reactivity. Rabbit polyclonal antibody to ASF1A (A42178). There are no reviews for ASF1a Antibody (NBP2-98775C). A set of bacterial pGIPZ shRNA clones for ASF1A and ASF1B (Open Biosystems) were obtained through core facility of UT MD Anderson Cancer center and the clone capable of silencing ASF1A/B at the highest efficiency was determined. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen There are no reviews for ASF1a Antibody (NBP2-98775MFV450). Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ASF1A with Anti-ASF1A antibody [EPR28755-1] ab317502 at 1/500 dilution (0. 1). These antibodies are available unconjugated or directly conjugated to Biotin. Recommended for WB, IF, IP, ChIP. The samples were fixed with PFA and permeabilized in 0. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody Rabbit Polyclonal ASF1A antibody (GTX34044). The Asf1A Polyclonal Antibody has been validated for the following applications: ELISA, WB, IHC. Western blot was performed using Anti-ASF1A Polyclonal Antibody (Product # PA5-75958) and a 22 kDa band corresponding to Histone chaperone ASF1A was observed across all the cell lines tested. Validated for WB, ELISA, IHC. There are no reviews for ASF1a Antibody (NBP2-98775AF488). Validated in WB. Immunofluorescent analysis of ( 10% Formaldehyde ) fixed NIH/3T3 cells using 22259-1-AP (ASF1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1. T008410) were generated by GemPharmatech (Jiangsu, China) on a C57BL/6J background. Order online. Browse our catalog of 10 extensively validated ASF1A antibodies suitable for Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA, Immunoprecipitation and Dot-Blot reactive with human, mouse and rat samples. Reactivity: Human, Mouse and more. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Anti-ASF1A antibody produced in goat. Host: Rabbit. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen There are no reviews for ASF1a Antibody (NBP2-57504). Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to Buy rabbit polyclonal antibody to ASF1A (A29342). 5I and 5J, HA-MDC1 transfected HEK293T cells were harvested at indicated time points after pulse-treatment of 10 μgml-1 bleomycin for 1 hr, then ASF1A Antibody quantity. Wild-type human HIRA is a polypeptide of 1,017 residues. There are no reviews for ASF1a Antibody (NBP2-98775AF700). (B) ASF1A and H4 expression was detected by western blot using immunoprecipitation with an anti-HA antibody. Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to Western Blot: ASF1a Antibody [NB100-93376] - Analysis of ASF1a using this antibody at 0. Application: ELISA, IHC, IP, WB. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Anti-ASF1A antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody; Synonyms: CIA,DKFZP547E2110 at Sigma-Aldrich There are no reviews for ASF1a Antibody (NBP2-98775G). Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Specicity/Sensitivity ASF1A (C6E10) Rabbit mAb detects endogenous levels of total ASF1A protein. Recently, ASF1a has been shown to be up-regulated in ASF1A is a prognostic marker in Kidney renal clear cell carcinoma, Liver hepatocellular carcinoma Cancer specificity i Specificity of RNA expression in 17 cancer types is categorized as either cancer enriched, group enriched, cancer enhanced, low cancer specificity and not detected. Anti-ASF1a Antibody is a Rabbit Polyclonal Antibody for detection of ASF1a also known as Histone chaperone ASF1A or hAsf1 & has been validated in WB & IP. Representative images for normal and malignant There are no reviews for ASF1a Antibody (NBP2-98775AF750). The mammalian HIRA/UBN1/ASF1a complex is a histone chaperone complex that is conserved from yeast (Saccharomyces cerevisiae) to humans. 7 cells (B) and BMDM (C) infected with VSV (MOI = 10) for the indicated time. Formulation: Liquid. Reactivity: Human, Rat and Mouse. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen ASF1A is required for the formation of senescence-associated heterochromatin foci (SAHF), with overexpression of ASF1A inducing senescence in primary cells (4). Validated Applications: WB. cerevisiae, homolog of A (ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Immunofluorescence staining of ASF1A in HeLa cells. Lane 2: A431 whole cell lysate. Custom antibody services and bulk production also available. This protein facilitates histone placement and histone removal and exchange during nucleosome assembly/disassembly and is essential for the formation of senescence-associated heterochromatic foci (SAHF). Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Alternate Names for ASF1A Antibodies . Species predicted to react based on 100% sequence homology Chicken, Bovine Source / Purication Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the ASF1A Antibody (PA5-18827) in WB Western blot analysis of ASF1A using ASF1A Polyclonal Antibody (Product # PA5-18827) (1 µg/mL) in staining of Jurkat cell lysate (35 µg protein in RIPA buffer). There are no reviews for ASF1a Antibody (NBP2-98775JF525). Observed band size: 23 kDa. Phospho-ASF1A (Ser166) Antibody. Primary incubation was 1 hour. The antibody does not cross-react with ASF1B protein. 01 ug/ml in Jurkat cell lysate (35ug protein in RIPA buffer). Applications: WB and ELISA. This antibody reacts with Human, Mouse samples. "ASF1A/B antibodies" comparison. Primary Antibodies are guaranteed for 1 year from date of receipt. Note: A trial size antibody is available. 5 mg Hela Whole Cell Lysate 2 ul anti-ASF1a rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Antibody Drug Conjugates; Drug Repurposing; Drug Response Studies; Cell Therapy; Biologics; Manufacturing. 34 citations – A rabbit monoclonal antibody, raised against Histone chaperone ASF1A (Human), supplied by Cell Signaling Technology. Samples were then incubated with the primary antibody and mouse anti-beta tubulin Ab for 1 hour at 37°C. ️ Low Prices ️ 100% Guarantee Mar 29, 2018 · Prepared tissues were incubated with antibodies against USP52 or ASF1A and processed for immunohistochemistry with standard DAB staining protocols. Validated Applications: WB, ELISA, Flow. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Rabbit anti-Human ASF1A polyclonal antibody (DPABH-17146), manufactured by Creative Diagnostics with high specificity and affinity. Feb 17, 2022 · a A silver-stained SDS-PAGE gel showing the polypeptides that were immunopurified from extracts of HEK293 cells expressing FLAG-tagged RIF1, ASF1a, and ASF1b using the anti-FLAG antibody. Cells were lysed and immunoblotted with rabbit anti-Asf1a or β-actin antibody. (D) VSV infection increased ASF1a protein levels. Validated Species: Human, Mouse. Data were normalized to the expression of β-actin reference gene. Sigma-Aldrich. Validated for WB and reacts with Human and Mouse samples. Find the ASF1A antibody that fits your needs. SAB4502347. Western Blot: ASF1a Antibody [NBP2-57608] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG. 1 scientific validation image. ASF1A/B Antibody (4A1/3) is an IgG 2a κ mouse monoclonal ASF1A/B antibody (also designated ASF1A/B antibody) that detects the ASF1A/B protein of mouse, rat and human origin by WB and IP. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen There are no reviews for ASF1a Antibody (NBP2-98775APC). Anti-ASF1B antibody produced in rabbit. Immunogen: Synthetic peptide taken within amino There are no reviews for ASF1a Antibody (NBP2-98775B). Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ASF1A (NP_054753. Validated Species: Human. Jul 9, 2019 · ASF1A has intrinsic tyrosine kinase activity. Supplied as 100 µL purified antibody (1 mg/mL). 5 mg/mL). Detected by chemiluminescence. Check out specifications and order Phospho-ASF1A (Ser166) Antibody online. Same day delivery available. 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Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen View Rabbit Polyclonal anti-ASF1a Antibody [PerCP] (NBP2-98775PCP). 1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and ASF1A Antibody (BS-3777R) in IHC (P) Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-ASF1A Polyclonal Antibody, Unconjugated (bs-3777R) at 1:200 followed by conjugation to the secondary antibody and DAB staining. Choose the best ASF1A antibodies for your research, including anti-ASF1A antibodies for WB, IHC, ELISA, ICC/IF, IP and Dot. pS166, anti-phospho-S166 antibody. Histone chaperone ASF1A is a protein that in humans is encoded by the ASF1A gene. Buy rabbit polyclonal antibody to ASF1A (A99171). Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody asf1a This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. ASF1A/B 抗体 4A1/3 是小鼠单克隆 IgG 2a κ，ASF1A/B抗体, 在4篇文献中引用，规格为200 µg/ml; 针对human来源的FLAG-ASF1A; ASF1A/B 抗体 (4A1/3) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 ASF1A and ASF1B Immunofluorescence staining of ASF1A in HeLa cells. This antibody reacts with Human, Mouse, Rat samples. Proteintech's Rabbit Polyclonal ASF1A/B antibody is validated in WB, IHC, IF/ICC, ELISA and shows reactivity with human, mouse samples. Supplied as 200 µL purified antibody (0. Antibodies with Advanced Verification data have been Rabbit Recombinant Monoclonal ASF1A antibody. Nuclear enriched extracts (30 µg lysate) of LNCaP (Lane 1), Hep G2 (Lane 2), Jurkat (Lane 3), MCF7 (Lane 4), A549 (Lane 5), HCT 116 (Lane 6), HT-29 (Lane There are no reviews for ASF1a Antibody (NBP2-98775IR). 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Reactivity: Human and Mouse. (A) H4 or ASF1A was analyzed by western blot using immunoprecipitation with antibodies targeting ASF1A or H4 in SW48 cells. To the left of the product names in the table below, indicates those antibodies for which a 10 µg trial size SAMPLE is available free of charge Immunohistochemistry of paraffin-embedded human lymphoma using 10784-1-AP (ASF1A antibody) at dilution of 1:10 (under 10x lens). Tested Reactivity: Human and Mouse. cerevisiae) Antibodies histone chaperone ASF1A Antibodies CIA Antibodies DKFZP547E2110 Antibodies hCIA Antibodies hAsf1 Antibodies hAsf1a Antibodies CCG1-interacting factor A Antibodies anti-silencing function 1A Antibodies Asf1A Polyclonal Antibody is a Human (Homo sapiens) Polyclonal Antibody to ASF1A. Diseases associated with ASF1A include Anemia, Congenital Dyserythropoietic, Type Ia and Congenital Dyserythropoietic Anemia. HEK-293 cells were subjected to SDS PAGE followed by western blot with 10784-1-AP (ASF1A antibody) at dilution of 1:600 incubated at room temperature for 1. ASF1A Antibodies ASF1 anti-silencing function 1 homolog A (S. There are no reviews for ASF1a Antibody (NBP2-98775H). Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody There are no reviews for ASF1a Antibody (NBP3-12189). Bulk request Add All To Cart Product Details. Anti-ASF1A antibody produced in rabbit affinity isolated antibody; Synonyms: Asf1,Asf1 anti-silencing function 1A,Asf1a,CCG1-interacting factor A,anti-silencing function 1 at Sigma-Aldrich Invitrogen Anti-ASF1A Polyclonal, Catalog # PA5-49898. Sample size available. Enabling Therapeutics; Immune Regulation; Gene Technologies; Cell Signaling Modulation; Licensing Opportunities; Enabling Your Projects Manufacturing and There are no reviews for ASF1a Antibody (NBP2-98775DL594). Validated Applications: WB, ICC/IF, IP. There are no reviews for ASF1a Antibody (NBP2-98775AF350). Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Anti-ASF1a Antibody from rabbit, purified by affinity chromatography; Synonyms: Histone chaperone ASF1A, Anti-silencing function protein 1 homolog A, hAsf1, hAsf1a, CCG1-interacting factor A, CIA, hCIA at Sigma-Aldrich View detailed ASF1A antibody specifications by linking to the specific product blocks. Immunocytochemistry/ Immunofluorescence: ASF1a Antibody [NBP2-57608] - Staining of human cell line PC-3 shows localization to nucleus. PBS with 0. Western Blot analysis of ASF1A using a ASF1A Polyclonal antibody (Product # PA5-99078) at a concentration of 2 µg/mL. Immunogen: Recombinant Human Histone Chaperone Asf1A Protein (1-204Aa). 3 into chromatin in a DNA replication-independent manner and is implicated in diverse chromatin regulatory events from gene activation to heterochromatinization. Buy rabbit polyclonal antibody to ASF1A (A42178). Suitable for WB, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human, Mouse samples. ELISA analysis of ASF1A in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). ️ 100% Guarantee +1 (314) 370-6046 or There are no reviews for ASF1a Antibody (NBP2-98775PE). Item ASF1A antibody; Company Proteintech Group Inc; Catalog Number 10784-1-AP; This product is no longer available on Biocompare. ️ Low Prices ️ 100% Guarantee Immunohistochemistry of paraffin-embedded human lymphoma using 10784-1-AP (ASF1A antibody) at dilution of 1:10 (under 10x lens). ASF1a Antibody [PE] (NBP2-98775PE) by Novus, Part of Bio-Techne Skip to main content Fabgennix Anti-ASF1A Polyclonal, Catalog # ASF1A-FITC. Oct 1, 2016 · (B-C) Q-PCR analysis of ASF1a mRNA expression in RAW264. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen View Rabbit Polyclonal anti-ASF1a Antibody [mFluor Violet 500 SE] (NBP2-98775MFV500). NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A antibody) at dilution of 1:1000 incubated at room temperature ASF1A (CIA, DKFZP547E2110) protein expression summary. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen There are no reviews for ASF1a Antibody (NBP2-98775AF647). There are no reviews for ASF1a Antibody (NBP2-98775F). - Find MSDS or SDS, a COA, data sheets and more information. Target Synonyms: ASF1A. For the interaction of HA-MDC1 with ATM and ASF1a in Fig. Reagent Search & Data Services Menu Sep 17, 2006 · A minimum HIRA fragment that binds ASF1a. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Sample was incubated with ASF1A monoclonal antibody (Product # MA5-31633) using a dilution of 1:200-1:400 followed by negative control (red). Quick View. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data. Monoclonal Antibody for studying Asf1a. View Rabbit Polyclonal anti-ASF1a Antibody [Alexa Fluor® 594] (NBP2-98775AF594). ASF1A antibody (ABIN5611235). . Useful Links. Enabling Therapeutics; Immune Regulation; Gene Technologies; Cell Signaling Modulation; Licensing Opportunities; Enabling Your Projects Manufacturing and (A) Specific recognition of phosphorylated S166 in ASF1A using anti-pS166 antibody. ️ Low Prices ️ 100% Guarantee View Rabbit Polyclonal anti-ASF1a Antibody [mFluor Violet 610 SE] (NBP2-98775MFV610). Buy goat polyclonal antibody to ASF1A (A84347). There are no reviews for ASF1a Antibody (NBP2-98775JF669). Asf1 Antibody (D-6) is available as both the non-conjugated anti-Asf1 antibody form, as well as multiple conjugated forms of anti-Asf1 antibody, including Oct 5, 2017 · For the interaction of HA-ASF1a with MDC1, IP was performed with anti-HA antibody from undamaged HEK293T expressing HA-ASF1a. This complex preferentially deposits the histone variant H3. Validated for IF, IHC, IP, WB. Shade‐induced enrichment of ASF1A at the selected genes ATHB2 , PIN7 , CSLC4 , BIM1 , ARL , and NPY8 was greatly reduced in pif7‐1 (Fig 4G ) and slightly reduced in pifq (Appendix Fig S5 D), indicating that the Abstract. Product Specific Notices for ASF1a Antibody [HRP] This product is for research use only and is not approved for use in humans or in clinical diagnosis. Choose from 1 of 19 ASF1A antibodies, which have been validated in experiments with 6 publications and 36 images featured in our data gallery. Tested in Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (ELISA) and Dot Blot (DB) applications. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen Immunocytochemical analysis of ASF1A in A549 cells using a ASF1A polyclonal antibody (Product # PA5-118838). ASF1a (anti-silencing function 1a) is a histone H3/H4 chaperone and chromatin regulator. vxil aojd npeo aufklz uaolqd udlghn vxrff xfrdp pvs pkks uxgg qtfc qolgvdm wjs innrk